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Developing a UHPLC method for UV-based detection and quantification of primary aromatic amines in low concentrations
6 Aug 2020This application note by Agilent demonstrates that stringent sensitivity requirements for the detection of potentially harmful primary aromatic amines can be fulfilled.
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Agilent 1290 Infinity III Diode Array Detector FS
Agilent TechnologiesThe 1290 Infinity III Diode Array Detector FS (fixed slit) delivers multiple wavelength detection with full spectra at sampling rates up to 120 Hz for fast LC separations. The Max-Light cartridge cell features optofluidic waveguides, which improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.