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Determination of Quantitative Protein Signatures for Ductal Carcinoma (Breast Cancer) by LC/MS Proteome Analysis

Waters
4 Feb 2015

In this study, a comprehensive MS-based discovery strategy is applied for a polygenic disease. The method employs the separation and detection of non-labelled tryptic fragments by means of an LC/MS acquisition. Instrumentation used included the Waters Expression High Definition Proteomics System, made up of the nanoACQUITY UPLC System and the Q-Tof Premier Mass Spectrometer.

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IdentityE High Definition Proteomics system

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The Waters IdentityE High Definition Proteomics system defines the new standard for rigorous protein identification. The IdentityE High Definition Proteomics System is an UltraPerformance LC® (UPLC)/High-Bandwidth Mass Spectrometry (MS E) system solution suitable for confident protein identification designed to meet stringent requirements as outlined by leading scientific journals.The new Waters IdentityE High Definition Proteomics System catalogs complex protein digest mixtures containing tens of thousands of peptides. The system features a Waters nanoACQUITY® UPLC System for high chromatographic peak capacity and retention time reproducibility, together with an electrospray tandem mass spectrometer - Waters Q-Tof Premier™ or Waters Synapt HDMS system - for high dynamic mass resolution and consistent over-sampling of complex protein digests – two hallmarks of high definition proteomics. Waters comprehensive peptide ion accounting informatics mine deeper into UPLC/MS E data to identify more peptides/proteins with greater sequence coverage and statistical rigor than conventional LC/MS/MS methods. This system solution is fully supported with quality control standards, defined methods, training and support to ensure reproducible operation and consistent results.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Breast CancerBreast cancer is a type of cancer that begins in the cells of the breast and can spread to other parts of the body. Early detection, accurate diagnosis, and targeted treatments are essential for improving patient outcomes. Browse our peer-reviewed product directory to find the best breast cancer research and diagnostic tools, compare products, check reviews, and get pricing directly from manufacturers.

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