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Determination of multiple quality attributes of antibody-oligonucleotide conjugate with SEC-MALS and AEX-MALS
3 Feb 2025Multi-angle light scattering coupled to liquid chromatography (LC-MALS) can be used to analyze antibody-oligonucleotide conjugates (AOCs), providing a robust and straightforward approach to obtaining multiple quality attributes. Wyatt Technology™ demonstrates the successful application of LC-MALS with both size-exclusion chromatography (SEC) and anion exchange chromatography (AEX) to quantify the oligonucleotide-antibody ratio (OAR) and overall payload concentration of two AOC samples. Explore how through a single LC-MALS experiment, the complex molecular species present in the samples, was identified, including aggregates, fragments, and different OAR populations.
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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.