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Determination of K<sub>m</sub> for ATP and Kemptide with Protein Kinase A Enzyme using Transcreener® ADP<sup>2</sup> FP Assay
4 Nov 2014The Transcreener® ADP2 assay is a simple one-step homogenous detection assay. This application note describes a method for the determination of Km for ATP and Kemptide with Protein Kinase A Enzyme using Transcreener® ADP2 FP Assay.
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Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.
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