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Determination of Carbohydrates in Urine by HPAE-PAD
23 Oct 2014In this application note two high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) methods are compared for the analysis of urine samples. The first method, using a Thermo Scientific Dionex CarboPac PA20 Analytical Column minimizes eluent preparation with a Reagent-Free™ ion chromatography (RFIC™) eluent generator. The second method discussed takes advantage of the selectivity and high capacity of the Dionex CarboPac MA1 column. Both methods are shown to successfully determine carbohydrates in urine with good precision and minimal matrix effects from samples.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GlycobiologyGlycobiology encompasses research into the biological functions, synthesis and structures of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides. Mass spectrometers and their software are commonly used in the study of glycobiology, along with structure predicting tools, chromatography and NMR.CarbohydratesHPAE-PAD