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Detection of Vancomycin-Induced Subacute Nephrotoxicity Using MILLIPLEX<sup>®</sup> Map Rat Kidney Toxicity Multiplex Panels

Merck KGaA, Darmstadt, Germany
5 Jun 2016

This application note presents a study into a panel of urinary biomarkers, investigating how they perform after 28 days of treatment with a model nephrotoxic compound, vancomycin8. This compound is a glycopeptide antibiotic that has been previously shown to affect primarily the tubules in cases of vancomycin-induced kidney injury.

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ISOSLIDE® Reticulin Control Slides for the detection of reticular fibers in histology

Merck KGaA, Darmstadt, Germany

To ensure that the test preparations contain the material relevant for diagnostics and the target structures can be visualized by staining with good differentiation, it is recommended to use a control preparation. ISOSLIDE® control slides enable a direct comparison of typical control material with laboratory-internal specimens.Reticular fibers consist of thin bundles of fine fibrils of type III collagen. These collagen fibrils react readily with silver salts and are stained black following silver plating. One ISOSLIDE® Reticulin kit contains: Control Slides with reference tissue for the detection of reticular fibers in histological tissue, 24 unstained paraffin sections, 1 stained control slide with Reticulin silver plating according to Gordon & Sweets. Your own samples can be compared with the control tissue specimens All slides prepared from the same block of animal tissue (one Box contains one Batch) Five-year shelf life Compliant with ISO 9001 and CE certification in accordance with in vitro diagnostic medical devices (IVD) guidelines How to use the ISOSLIDE® control slides?The ISOSLIDE® control slides consist of microscope slides with a paraffin section containing the typical structures of the test method to be used. Each pack contains 25 slides: One slide is stained with the reference method and is used for comparison, the other 24 unstained slides are stained according to the corresponding Merck Millipore specification or else can be stained to the laboratory-internal specification. The unstained control slides are stained and subsequently compared with the laboratory material and the stained control section regarding the staining result.

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DPX New mounting medium - water-free, less hazardous

Merck KGaA, Darmstadt, Germany

DPX new – without teratogenic DBP plasticizer for use in histology processes with xylene. In histology, users are in frequent contact with hazardous reagents. During fixation, histoprocessing, staining and mounting, laboratory technicians and pathologists are surrounded by solvents and solvent-containing solutions. These can cause health problems, as they may, for example, be carcinogenic or teratogenic. Di-N-Butyl Phthalate in Xylene (DPX) new no longer contains the ingredient Dibutyl phthalate (DBP), which is teratogenic and normally used as plasticizer.Further development of well-established mounting medium DPX. No admixture of the teratogenic ingredient DBP, used as a plasticizer: Lower health risk for users in histology laboratories. Refractive index and viscosity are comparable to DPX mounting medium.

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Detection of Vancomycin-Induced Subacute Nephrotoxicity Using MILLIPLEX<sup>®</sup> Map Rat Kidney Toxicity Multiplex Panels