ResourceSeparations

Detection of Nordiazepam and Oxazepam in Calliphora Vicina Larvae using LC/MS/MS

16 May 2012

In this study LC/MS/MS is used to detect the Benzodiazepine Nordiazepam and its metabolite Oxazepam, in single larvae of the Calliphora vicina. Benzodiazepines are prescribed for the symptomatic treatment of anxiety and sleep disorders. They are frequently encountered in postmortem blood analysis (suicide or accidental deaths).

Alliance® HPLC - e2695 Separations Module

Waters

The Waters Alliance 2695 Separations Module is a high performance liquid chromatographic system with a quaternary, low-pressure mixing pump and inline vacuum degassing. Flow rates from 50 uL/min to 5 mL/min can be generated for use with 2.1 mm ID columns and larger. The autosampler has a maximum capacity of 120 vials (12x32, 2-mL) with programmable temperature control from 4 to 40°C. A heated column compartment provides temperatures from 5 degrees above ambient to 65°C. The detector is a photodiode array (model 2996) with a wavelength range of 190-800 nm and sensitivity settings from 0.0001-2.0000 absorbance units. All components of the HPLC are controlled through Waters Empower software.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.BenzodiazepinesOxazepamNordiazepam
Detection of Nordiazepam and Oxazepam in Calliphora Vicina Larvae using LC/MS/MS