ResourceLife Sciences
Detection of HMT G9a with the Transcreener® EPIGEN Methyltransferase Assay
9 Sept 2015The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. This Application note provides a guide for using the Transcreener® EPIGEN ethyltransferaseAssay to detect the initial velocity enzyme activity of HMT G9a with an assay window suitable for inhibitor screening and dose response measurements.
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