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Detection of HIF1α Expression at Physiological Levels Using NanoLuc® Luciferase and the GloMax® Discover System

Promega Corp.
2 May 2017

This application note demonstrates the utility of NanoLuc® Luciferase and the GloMax® Discover System for measuring gene expression at physiologically relevant levels – using hypoxia inducible factor 1 alpha expression (HIF1α) as an experimental system. It also shows that the demonstrate detection with the GloMax® Discover System. It finds that the GloMax® Discover System coupled with the NanoLuc® luciferase technolog

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GloMax® Discover Microplate Reader

Promega Corp.

  This ready-to-use multimode detection system includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.   The GloMax® Discover System is a high-performance multimode detection instrument developed with Promega reagent chemistries to provide a simple means of detecting advanced chemistries. This instrument provides researchers superior luminescence sensitivity and dynamic range for both strong and weak bioluminescence experimental samples, as well as a seamless integration with Promega’s industry leading bioluminescent assays. GloMax® Discover also provides flexible use of filters for fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance measurements. The instrument is operated by an integrated Tablet PC, which provides quick and easy navigation through the control options. Exporting your results is made seamless with a variety of options, including exporting to your local data network. The GloMax® Discover software provides the required technical elements of a 21 CFR Part 11-compliant system to be used with the appropriate laboratory workflow. GloMax® Discover System Features: Integrated with Promega Assays - Developed and optimized with Promega cell and gene reporter assays for seamless workflow; you will be up and running faster. Easy-to-Use - Simple Tablet PC touch screen navigation with full PC capabilities and a state-of-the-art graphical user interface. Superior Performance - Broader dynamic range, better sensitivity and lower well-to-well cross talk for more usable data from your experiment. Cost-Effective - Spend less time optimizing. Connected to Your Workflow - Use as a standalone instrument or integrate into your high-throughput automated workflow; export data to your laboratory network; enable 21 CFR, Part 11 electronic signature compliance. Applications: Integration with Promega reagent chemistries for detection of fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance assays. Integration into low- and medium-throughput automation workflows. Optimization with Promega cell and gene reporter assays.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.BioluminescenceReporter AssayAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.HypoxiaLuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.

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Detection of HIF1α Expression at Physiological Levels Using NanoLuc® Luciferase and the GloMax® Discover System