Detection of Amino Acids Using HPLC with CD Detection
Detection of Amino Acids Using HPLC with CD Detection
6 Jan 2016This application note will describe chiral detection of amino acids using HPLC. Amino acids are often described as the “building blocks” of the body. The twenty naturally occurring amino acids that comprise proteins are almost all of the L- form. The L (Levorotatory) form is the stereoisomer that rotates plane polarized light to the left. One method to detect amino acids and determine their stereochemistry is with HPLC.
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LC-4000 Circular Dichroism Detector
JASCO (USA)The CD-4095 is a unique HPLC detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter.Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column. The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring the positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.