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Detection and Quantification of Leached Protein A from Eshmuno® A Resin Using Cygnus F400 Protein A ELISA kit

Merck
23 Oct 2014

This case study demonstrates the performance of the Cygnus Protein A ELISA Kit with samples containing EMD Millipore’s Eshmuno® A Protein A ligand. The initial feasibility testing demonstrates that the Cygnus F400 Protein A ELISA Kit could accurately measure the Eshmuno® A Protein A ligand. In phase II, the accuracy and precision of the ELISA was assessed using samples from a 3-step antibody purification process. The F400 Protein A ELISA Kit performed within the generally accepted specifications. Based on this testing, the Cygnus F400 Protein A ELISA is qualified for measuring the Eshmuno® A Protein A ligand in a typical downstream process using Eshmuno® A resin. Eshmuno® A resin has a high binding capacity, superior flow capability and excellent batch-to-batch consistency so increases productivity and reliability of results.

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Eshmuno® A Resin

Merck

Eshmuno® A is a rigid, high capacity Protein A affinity chromatography resin for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies. Eshmuno® A resin can be cleaned and sanitized under acid and alkaline conditions while maintaining high dynamic binding capacity at high flow rates.The Eshmuno® A resin contains a EMD Millipore proprietary ligand. This ligand is recombinantely expressed in E. coli. No animal derived products are used during production. The Eshmuno® A resin is synthesized via immobilization of the ligand on to the Eshmuno® base matrix, which is a rigid and hydrophilic polymer based on polyvinylether.Eshmuno® A Resin Features: Productivity - high binding capacity, superior flow capability, reusability, and low cost of goods (COGS). Product purity - increased aggregate removal, excellent HCP reduction, low leached Protein A, consistent viral clearance, and reduced Fab binding. Scalability - linear scale-up. Reliability - batch-to-batch consistency, GMP manufacturing environment, and continuous regulatory support.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.

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