Detecting Cancer Burden in Liquid Biopsies

12 Apr 2017

Liquid biopsy is a non-invasive sample source that can be utilized to assess cancer burden by measuring the tumor-derived fraction of circulating, cell-free DNA (cfDNA) from plasma. The study detailed on this poster evaluates two methods to monitor cancer burden using cfDNA: whole genome bisulfite sequencing (WGBS) and targeted amplicon sequencing for 56 oncology-related genes.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. 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Detecting Cancer Burden in Liquid Biopsies