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Detailed characterization of mitosis using cell population analysis and auto ROI defined confocal imaging

Agilent Technologies
10 Dec 2021

This application note from Agilent demonstrates the use of the Auto ROI feature of Gen5 to identify mitotic cells for further characterization using confocal microscopy performed by the Cytation C10 confocal imaging reader.

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Agilent BioTek Cytation C10 Confocal Imaging Reader

Agilent Technologies

The Cytation C10 confocal imaging reader combines automated confocal and widefield microscopy with conventional multimode microplate reading in a unique, proprietary design. The automated water- immersion objectives capture more light, driving lower exposure times and reducing phototoxic impacts on live cells. The spinning-disk confocal module and accompanying disk pinhole options allow a researcher to look deeper into thick sample biology with improved clarity and detail. High-quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality, at a significantly lower price than others. Cytation C10 also includes widefield fluorescence, brightfield and phase contrast optics. Onboard environmental controls enable live cell assays. Multiplate live cell assays are accomplished with Cytation C10 integrated with the Agilent BioTek BioSpa 8 automated incubator. Cytation C10 brings high-performance, affordable confocal to every laboratory.

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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.ConfocalImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.

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Detailed characterization of mitosis using cell population analysis and auto ROI defined confocal imaging