Deep structured illumination microscopy of neurobiological samples

Widefield (WF) and confocal (CF) microscopy might be sufficient to study cellular and tissue morphology and dynamics but are often not enough to accurately answer all biological questions. Getting a further level of information could allow new discoveries and, in this regard, super-resolution (SR) microscopy has become an increasingly popular and robust tool across life sciences to study fine cellular structures and sub-cellular components, as well as their dynamic processes. CrestOptics are working to make SR accessible to all researchers and to advance the potential of their scientific discoveries, moving them to a next level. Its newest product, the DeepSIM, was developed with this purpose and it is the first SR module based on structured illumination compatible with any existing upright or inverted microscope with a camera port, it is as easy to use as a CF microscope, and it enables scientists to access deep data about their biological samples. The DeepSIM is designed to work with samples of thickness comparable to those used in CF microscopy and in this application note, CrestOptics focuses on super-resolved deep imaging of different neurobiological samples at high magnification.