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Decoding disease epigenetics: Innovating DNA methylation with advanced sequencing methods

Agilent Technologies
2 Jul 2025

Explore how the Functional Epigenetics Laboratory at KU Leuven in Belgium has integrated the Agilent Fragment Analyzer system into its workflow to maximize efficiency, especially during single-cell nucleosome, methylome, and transcriptome sequencing (scNMT-seq). This technique allows for the simultaneous analysis of chromatin accessibility, DNA methylation, and gene expression within individual cells.

Discover how the lab also uses the Agilent HS NGS Fragment kits with the Fragment Analyzer due to the high-sensitivity analysis, which is crucial for NGS library QC from limited DNA amounts. The libraries often contain minimal DNA after the scNMT-seq protocol, making these kits essential.

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Fragment Analyzer Systems

Agilent Technologies

The Agilent Fragment Analyzer systems are fluorescence-based capillary electrophoresis instruments for the accurate sizing, quantification, and qualification of nucleic acids (DNA and RNA).

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Capillary ElectrophoresisCapillary electrophoresis (CE) is used to separate molecules based on charge, size and hydrophobicity. Pressure, voltage or a vacuum is used to introduce the sample to fused silica capillaries. Fluorescence, UV/Vis, UV or diode array detectors are used to visualize components. Types of CE systems include capillary electrochromatography (CEC), capillary zone electrophoresis and capillary gel electrophoresis. Find the best capillary electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.EpigeneticsEpigenetics refers to heritable changes in gene expression. These changes are not caused by alterations to the DNA sequence. Three of the main systems responsible for the initiation of epigenetic change are DNA methylation, histone modification and non-coding RNA-associated gene silencing.

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Decoding disease epigenetics: Innovating DNA methylation with advanced sequencing methods