ResourceLife Sciences
CyTRAK Orange in Live End-Point Imaging
14 Mar 2019In fluorescence-based microscopy, it is normal to preserve cells or tissue sections with formaldehyde or a similar fixative. However, there are certain problems associated with fixing, such as leakage, and suitability for studying drug-receptor interactions in cells, or other live-cell end-point assays. In this application note, it is shown how cell-impermeant DNA dyes are unusable as nuclear counterstains in such assays, but the orange fluorescing live-cell-permeant DNA probe CyTRAK orange provides clear nuclear counterstaining as a feasible alternative.
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In Vivo Imaging Systems<i>In vivo</i> imaging systems, including pre-clinical imaging systems and medical imaging systems are used to non-invasively visualize and capture images of live animals and plants. Monitor the natural processes or diseases of your subjects using small-animal pre-clinical imaging systems, including single photon positron emission tomography (SPECT), positron emission tomography (PET), computed tomography (micro-CT), magnetic resonance imaging (MRI), X-ray radiography, ultrasound, fluorescence and bioluminescence imagers. Multimodal systems and software solutions are also available for correlative analysis of organ, tissue, cell, or molecular-level processes. Find the best in vivo imaging products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Dyes