ResourceLife Sciences
Cytokine Antibody Array Applications with UVP BioImaging Systems
25 Feb 2016All cell functions, including cell proliferation, cell death and differentiation, as well as maintenance of health status and development of disease, are controlled by many genes and signaling pathways. New techniques such as cDNA microarrays have enabled the analysis of global gene expression. However, almost all cell functions are executed by proteins, which cannot be studied by DNA and RNA alone. This application note demonstrates an innovative protein array system with allows the simultaneous detection of multiple cytokine expression levels from conditioned media, patient’s sera and other sources
Links
Tags
ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AngiogenesisApoptosisApoptosis, or programmed cell death, is a vital process for healthy tissues and is key to our understanding of disease mechanisms like cancer and neurodegeneration. Research tools enable apoptosis detection, quantification, and analysis. Browse products for apoptosis research in our peer-reviewed product directory.CytokinesCytokines are signaling proteins critical for cell communication in the immune system. They regulate inflammation, immune responses, and cell growth. Cytokine research is vital for understanding disease mechanisms and developing therapies. Explore tools for cytokine detection and analysis in our directory.Gene ExpressionCardiologyStem Cell DifferentiationCell ProliferationCell proliferation refers to the process by which cells divide and multiply. This is a key concept in cancer research, tissue regeneration, and immunology studies. Explore cell proliferation tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.BioimagingCancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.