CyGEL Sustain™ in Image-Based Apoptosis Assays: Extended Immobilization of Non-Adherent / De-Adhering Cells

Microscopy offers the ability to gain important morphological information, including nuclear appearance to identify apoptotic events, or even subcellular location of other markers of interest. Cells undergoing death processes modify both localization and mobility properties of DNA-binding proteins and other compartmentalized and membrane‐located proteins allowing stages of apoptosis to be resolved. Sometimes there is a need to immobilize cells or even whole organisms over several hours to analyze events or signals of interest. This application note demonstrates how CyGEL Sustain™ provides a long-acting thermoreversible hydrogel mountant for non-adherent/motile cells.