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Culture medium analysis for a metabolic analysis of antibody-producing cells using LC-MS/MS and ICP-MS
1 Jun 2023This application note from Shimadzu describes how to use LC-MS/MS (LCMS-8060NX) to analyze 144 organic compounds and ICP-MS (ICPMS-2030) to analyze 9 inorganic elements in the culture medium and culture supernatant of a CHL-YN antibody producing cell line culture. This app note also describes how to use this data to identify which components and metabolic pathways are involved in antibody production.
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Nexera XS inert
Shimadzu CorporationThe Nexera XS inert system offers an ideal solution for the separation of biomolecules by combining the elevated pressure tolerance of a UHPLC system with complete inertness of the sample flow path, ensured by the absence of wetted metal surfaces and offering ultra-high resistance to corrosion.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ICP-MSInductively Coupled Plasma Mass Spectrometry (ICP-MS) is a powerful analytical technique used to determine the concentrations of elements and their isotopes in a variety of samples, including water, soil, and biological tissues. It involves ionizing elements in the samples with extremely high-temperature Argon (Ar) plasma and then using a mass spectrometer to measure the number of ions based on their mass-to-charge ratio (m/z). Explore ICP-MS systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.