Culture and Assay Systems Used for 3D Cell Culture

Culture and Assay Systems Used for 3D Cell Culture

2 May 2017

This application note will review the general principles, advantages and current limits of in vitro 3D cell culture systems, covering cell biology, tissue modelling and drug discovery and toxicity. The most commonly used matrices for in vitro 3D culture such as ECM-based hydrogels and their applications will also be covered. 3D cell culture methods have been developed over the years to address the limitations of 2D cultures, particularly their physiological irrelevance and inability to emulate in vivo conditions.

10-Layer CellCube® Module with 8,500cm² Growth Surface

Corning Life Sciences

The CellCube Module is an integral, encapsulated, sterile, single-use device that is 100% pressure-hold tested before shipment. It is comprised of a series of parallel, styrene plates joined to create thin, sealed, laminar flow spaces between adjacent plates. Each culture plate receives proprietary tissue culture treatment prior to assembly to ensure dense, uniform cell growth.These CellCube Modules provide:a series of parallel, polystyrene plates joined to create thin, sealed, laminar flow spaces between adjacent plates.8,500cm2 polystyrene growth surface optimally treated for the growth of attachment dependent cells.a perfused environment which more closely simulates in vivo conditions and reliably distributes nutrients and oxygen.an integral, encapsulated, sterile, single-use device that is 100% pressure-hold tested before shipment

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.In VitroIn vitro refers to experiments conducted outside living organisms, often in controlled lab environments such as petri dishes or test tubes. In vitro models are widely used in drug testing, cell biology, and disease research. Explore in vitro research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.Cell GrowthCell growth refers to the process by which cells increase in size and number. It is essential for tissue development, wound healing, and cancer research. Understanding and controlling cell growth is a fundamental part of cell biology and regenerative medicine. Browse our peer-reviewed product directory to find the best tools for studying cell growth, compare products, check reviews, and get pricing directly from manufacturers.Drug DiscoveryDrug discovery is the process of identifying potential new medications, involving stages such as target identification, compound screening, and preclinical development. It relies on cutting-edge technologies like high-throughput screening, artificial intelligence, and molecular modeling to accelerate the identification of drug candidates. Drug discovery plays a pivotal role in developing new therapies for diseases ranging from cancer to rare genetic disorders. Browse our peer-reviewed product directory to find the latest drug discovery technologies, compare options, check customer feedback, and get pricing directly from manufacturers.Cell Based Automation