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Critical Factors in Selecting an Internal Standard for Accurate Determination of Blood Alcohols in Post Mortem Samples

25 Feb 2013

Though use of n-Propanol as an internal standard for GC blood alcohol analysis is common, it can be difficult to resolve when analyzing post mortem samples. Further, n-Propanol can also be formed in the body’s natural decay process. This application note demonstrates how the Zebron™ ZB-BAC1 & ZB-BAC2 columns from Phenomenex were able to fully resolve both t-Butanol and 2-Butanol from other target analytes, while maintaining rapid analysis times of less than 2 minutes.

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Gas ChromatographyGas chromatography (GC) is an analytical technique used to separate and quantitate mixtures of small and volatile compounds. Gas chromatographs or GC systems include components such as GC columns, detectors, pumps and autosamplers. Choose from packed or capillary GC columns, flame ionization (FID), photoionization (PID) electron capture detectors and selective or non-selective detectors. Find the best gas chromatographs in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Evidence Collection and AnalysisEvidence Collection and Analysis is the retrieval and subsequent investigation into criminal evidence obtained from a crime scene. Forensic genetic instruments, kits and reagents enable the analysis of evidence collected  at the crime scene. Reagents and kits for presumptive analysis for body fluids such as blood, saliva and semen in addition to drugs and narcotics test kits are used for CSI. Fingerprinting equipment, forensic light sources, casting kits and equipment for collecting recoverable materials can be found in the directory.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.BAC TestingBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.Forensic InvestigationForensic investigation is a diverse collection of scientific techniques and methods for the identification and characterization of chemicals relating to crime scene investigations.
Critical Factors in Selecting an Internal Standard for Accurate Determination of Blood Alcohols in Post Mortem Samples