ResourceLife Sciences
Continuous Ion Current Recordings During Drug Delivery and Washout on an Automated Electrophysiological Platform
9 Jul 2011Here, Molecular Devices sought to determine whether the PatchXpress can be used to investigate the kinetics of slow recovery from compound block using the model of KV1.3 current following washout of three potassium (K+) channel blockers. Ionic currents activated during voltage clamp were recorded in CHO cells overexpressing KV1.3. To dissect the washout of agents in the immediate vicinity of the cell from actual interaction of the agents with the cell, the amplitude of the tail current was monitored by changes in the tail currents during the addition and washout of 45 mM extracellular K+.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. 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