SelectScience
Join Free
Accelerating Science
ResourceSeparations

Concanavalin A Column for Analysis of Glycoproteins and Their Tryptic Glycopeptides

Thermo Fisher Scientific
29 Aug 2012

This application note describes the how glycopeptides and glycoproteins can be efficiently isolated from complex samples using the Thermo Scientific HPLC-compatible ProSwift ConA-1S Affinity Column. The Thermo Scientific Dionex UltiMate 3000 x2 Dual Biocompatible Analytical LC system can be employed to automate the entire process. Identification of the glycopeptides captured by the ProSwift ConA-1S Affinity Column can be achieved by using a single quadrupole mass spectrometer.

Related products

Request Quote for All Products

Thermo Scientific™ ProSwift™ ConA-1S Affinty Column

Thermo Fisher Scientific

ProSwift™ ConA-1S Affinity Column for Glycan, Glycopeptide and Glycoprotein Enrichment and Purification The ProSwift ConA-1S affinity monolith column is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins from complex samples.The HPLC- compatible ProSwift ConA-1S affinity column maintains the specificity and selectivity for Con A binding glycans and glycoconjugates. The high capacity and ligand density of the ProSwift ConA-1S column facilitates the highly efficient enrichment of samples and can purify up to 2 mg of glycoproteins. The high peak efficiency of the column produces sharp, narrow peaks resulting in low elution volumes of highly concentrated products. The column is reusable, and many enrichments and purifications are possible with minimal loss of capacity.The ProSwift ConA-1S column, designed for and used in HPLC systems, provides many advantages compared to standard manual methods. These include faster separations, better enrichment and sample recovery, efficient washing capabilities, high peak efficiency and small elution volumes. Other advantages of the ProSwift ConA-1S column used with HPLC systems include automation, reusability, high-throughput capability and more accurate analysis with on-line monitoring. ProSwift™ ConA-1S Affinty Column Features: Highly efficient enrichment and purification High capacity and ligand density High sample recovery Low elution volumes Fast separations HPLC compatible Reusable Column Details: Chemistry: Concanavalin A (Con A) Length: 50 mm ID: 5 mm

(0)

Links

Thermo Fisher ScientificCompany website

Tags

AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. GlycobiologyGlycobiology encompasses research into the biological functions, synthesis and structures of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides. Mass spectrometers and their software are commonly used in the study of glycobiology, along with structure predicting tools, chromatography and NMR.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Post-Translational Modifications

Related resources

Concanavalin A Column for Analysis of Glycoproteins and Their Tryptic Glycopeptides