Comprehensive immunophenotyping of peripheral human whole blood

15 Mar 2023

Multiparameter flow cytometry is a powerful tool to monitor and characterize immune cell subsets. High-dimensional analysis of immune cells provides in-depth information on the state of the immune system at homeostasis or in response to stimulation. In this application note, learn more about a 24-color immunophenotyping panel for the five-laser Agilent NovoCyte Penteon flow cytometer which was designed for human peripheral blood to characterize the major immune cell subsets, providing a comprehensive overview of immune cell status.

NovoCyte Penteon Flow Cytometer

Agilent Technologies

The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte, and provides an expanded set of capabilities that accommodate today’s high-end and increasingly sophisticated multi-color flow cytometry assays. You now have the flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors. The NovoSampler Q can be integrated into different laboratory automation platforms and efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry-leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis, and reporting.  

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Flow CytometryBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.