ResourceLife Sciences
Comprehensive characterization of an antibody-drug conjugate using electron activated dissociation
26 Sept 2023In this application note from SCIEX, explore an in-depth characterization of a lysine-linked antibody-drug conjugate (ADC) using a streamlined, electron-activated dissociation (EAD)-based peptide mapping workflow in a single injection. This workflow combines the capabilities of EAD for peptide mapping with automated data interpretation offered by Biologics Explorer software. The workflow provides confident ADC sequence confirmation and accurate localization of the payload.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Antibody Drug Conjugates