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Comparison of SPE vs. SLE for the Quantitative Analysis of Anabolic Steroids in Serum Using LC-MS/MS

UCT, Inc.

Comparison of SPE vs. SLE for the Quantitative Analysis of Anabolic Steroids in Serum Using LC-MS/MS

23 Mar 2016

Analysis of anabolic steroids in serum typically requires a sample pretreatment step such as solid-phase extraction (SPE) prior to instrumental analysis. Traditional SPE-based methods utilize C18 or alternative reversed phase sorbents to retain the hydrophobic steroids. Alternatively, the use of a supported liquid extraction, or SLE, is also gaining popularity for this panel of compounds. This application note demonstrates a method that utilizes a traditional reversed phase interaction, but also features the addition of strong-anion exchange functionality (QAX) within the sorbent. This added functionality aids in the removal of unwanted matrix components commonly found in serum such as amino acids and inorganic ions.

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SELECTRA® HPLC Columns

UCT, Inc.

High purity, Type B, spherical silica support. 100A pore size. 1.8um, 3um, & 5um partical sizes. Variety of stationary phases.

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Clean-Up® Hydrophobic Columns

UCT, Inc.

These sorbents are composed of a silica backbone bonded with hydrocarbon chains. They are used to extract compounds, which exhibit non-polar or neutral characteristics, from complex matrices. The C18 phase is the most popular phase.  UCT offers a range of hydrocarbon chain lengths from C2 to C30. In addition, we offer isobutyl, tertbutyl, phenyl, and cyclohexyl bonded phases.

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Clean-Up® Hydrophilic Columns

UCT, Inc.

Compounds are retained on hydrophilic sorbents through polar interactions including hydrogen bonding, pi-pi or dipole-dipole interaction.  Compounds typically extracted on a hydrophilic column include analytes which have polar groups, including amines, hydroxyls and carbonyls. Elution is performed by strong polar solvents. For these types of extractions UCT offers various types of base silica, Florisil®, Alumina's, cyanopropyl and diol sorbents as well as non-porous graphitized carbon.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.Amino AcidsSolid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Anabolic SteroidMatrix EffectsHuman SerumHuman serum is found in blood plasma. The protein is used in a variety of applications including tissue engineering, transplantation and cell therapy.Steroids

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