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Comparison of protein sequencing analysis of CDNF, IL6, and FGF2 on Platinum and mass spectrometry

Quantum-Si
9 Feb 2024

In this application note, Quantum-Si discusses the challenges associated with traditional mass spectrometry (MS) methods for protein identification, including missed cleavages, chemical modifications, and the differentiation of similar amino acids. These factors often limit the accuracy of peptide mapping in proteomic analysis. Quantum-Si introduces its Platinum® instrument, which utilizes dye-tagged N-terminal amino acid recognizers and semiconductor chip technology to sequence proteins at the single-molecule level. This innovative approach overcomes the limitations of traditional MS methods by providing a user-friendly benchtop instrument and cloud-based analysis software, allowing researchers to achieve deeper proteomic insights without the need for expensive equipment or specialized expertise.

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Platinum® Next-Generation Protein Sequencer™

Quantum-Si

Easy-to-use, accessible benchtop single-molecule protein sequencing platform that empowers any lab, anywhere to obtain critical proteomic insights quickly and efficiently from the convenience of your lab, no expertise or infrastructure required.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NGSProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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