Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System

3 Jan 2017

To compare the behavior of cells in the RAFT™ 3D Cell Culture System, in contrast to the traditional 2D culture system, this application note describes the study of Human Bronchial Epithelial (HBE) Cells and Bronchial Smooth Muscle Cells (BSMC) from normal and asthmatic donors. In addition to analyzing mono-cultures of the two cell types in both systems, the RAFT™ 3D Cell Culture System allowed easy co-culture of the two cell types in a physiological orientation with the BSMC embedded in the RAFT™ Collagen and the HBE as a confluent cell layer on top of the BSMC-collagen matrix.

Links

Tags

Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.MuscleCell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.
Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System