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Comparison of ionization types and precursor ions for quantitative analysis of 25-hydroxyvitamin D on a TSQ Altis MD mass spectrometer

Thermo Fisher Scientific
10 Nov 2023

In this application note, Thermo Fisher Scientific compares variations of an analytical method for detecting and quantifying 25-hydroxyvitamin D2 and D3 using a Thermo Scientific™ TSQ Altis™ MD mass spectrometer. The note emphasizes the essential role of vitamin D in various biological functions, including promoting the absorption of calcium and supporting healthy bone growth. It highlights that vitamin D deficiency can lead to weakened bones, with manifestations like rickets in children and osteomalacia in adults, causing soft and malformed bones, reduced growth, and muscle weakness.

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TSQ Altis™ MD Mass Spectrometer

Thermo Fisher Scientific

Develop in vitro diagnostic tests for demanding quantitative analyses leveraging the enhanced sensitivity and remarkable speed and robustness of the Thermo Scientific™ TSQ Altis™ MD Series Mass Spectrometer. The TSQ Altis MD Series mass spectrometer, a Class I medical device, offers superb analytical performance and features the maximum usability, sensitivity and robustness required for laboratory developed tests (LDTs) from clinical diagnostic laboratories.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Vitamin D DeficiencyClinical Mass SpectrometryMass spectrometry is emerging as a superior method of analysis over the longstanding, gold standard of immunoassay. Clinical mass spectrometry systems are increasingly accessible to medical laboratories and are capable of providing results with more speed and less expense than traditional methods. From simple mass spectrometers to IVDR class A compliant, highly sophisticated systems performing liquid chromatography tandem mass spectrometry (LC/MS/MS), find the latest product news, reviews and resources to make a purchasing decision for your laboratory. Vitamin D

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