ResourceLife Sciences
Comparison of ELISA, Immuno-PCR, and SIMOA immunoassay methods
29 Jan 2025Enzyme-linked immunosorbent assay (ELISA) is the gold standard method for protein detection. This resource reviews three widely used ELISA platforms: standard ELISA, immuno-PCR (IQELISA®/BIQ-ELISA™), and single molecule array (Simoa®). These platforms are compared based on their design principles, sample volume requirements, instrumentation, and sensitivity.
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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.