ResourceLife Sciences
Comparison of Different Cell Types for Neutral Lipid Accumulation Using Image-Based Metrics to Quantify Lipid Accumulation in Cells
21 Nov 2018It has been postulated that liver cells accumulate neutral lipids as a response to stress from the exposure of free fatty acids. In vivo, the liver is the organ primarily responsible for the de novo synthesis of lipids, as well as the conversion of free fatty acids to neutral lipids by their esterification with glycerol. In vitro cell types other than liver cells can and do accumulate neutral lipids. This application note describes the use of the Cytation™5 Cell Imaging Multi-Mode Reader, to rapidly image and analyze a number of different lipid stained tissue culture cells.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.LipidsLipids are a diverse group of organic compounds that are insoluble in water, including fats, oils, and hormones. They are critical for cellular structure and energy storage. Explore lipid analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell CultureCell culture involves growing cells under controlled conditions, typically outside of their natural environment, to study cellular behavior, drug response, and disease models. Cell culture is crucial in biotechnology and biomedical research. Explore cell culture products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Hepatocytes