Comparing 3D optical microscopy techniques for metrology applications

22 Jun 2023

In this application note, Bruker Nano discusses the metrology advantages of Bruker’s ContourGT® 3D optical microscopes over confocal microscopes for certain applications. Key to these advantages is the ability to maintain subnanometer vertical resolution and 0.1 nanometer RMS repeatability, regardless of magnification or field of view.

3D optical microscopy is a mainstay metrology technology across a wide range of industries. There are a few techniques that provide a 3D surface representation from a microscope image, including the two key techniques of white light interferometry (WLI) and confocal microscopy, also known as laser scanning confocal microscopy (LSCM). These two methods are ubiquitous for measuring nanometers-to-millimeters surface heights. The principle of operation for each method provides different advantages and disadvantages.

ContourX-500

Bruker Nano Surfaces and Metrology

The ContourX-500 Optical Profilometer is the world’s most comprehensive automated benchtop system for fast, non-contact 3D surface metrology. The gage-capable ContourX-500 boasts unmatched Z-axis resolution and accuracy, and is easily customized for the widest range of complex applications, from QA/QC metrology of precision machined surfaces and semiconductor processes to R&D characterization for ophthalmics and MEMS devices.

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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Digital MicroscopyDigital microscopy involves using digital cameras and sensors to capture high-resolution images of samples for analysis. It offers enhanced imaging capabilities compared to traditional optical microscopy and is widely used in biological and material science research. Explore digital microscopy systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Confocal3D Imaging3D imaging technologies allow for the visualization and analysis of three-dimensional structures at high resolution. These systems are used in fields like molecular biology, material science, and medical diagnostics. 3D imaging can be applied to visualize cells, tissues, and organs, providing valuable insights into their structure and function. Browse our peer-reviewed product directory to find the best 3D imaging solutions, compare products, check reviews, and get pricing directly from manufacturers.MetrologyMicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.