ResourceLife Sciences
Co-Culture in ThinCert Cell Culture Inserts
19 Feb 2023Co-cultures with cell culture inserts are widely used to study mesenchymal-epithelial interactions during normal and tumoral development. In this application note such a co-culture model using MCF7 mammary carcinoma cells, human fibroblasts and ThinCert™ cell culture inserts from Greiner Bio-One GmbH is described. The results show that the use of ThinCert™ cell culture inserts with small pores (0.4 µm) guarantees that primarily paracrine signaling and not direct cell-cell contact accounts for the observed effects.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Paracrine SignalingCell-Cell Contactsco-culturemembrane inserts