ResourceSeparations

Chromolith® HPLC columns: Race Through Separations with Revolutionary Technology

21 Nov 2014

Due to their revolutionary monolithic technology, Merck Millipore’s Chromolith® HPLC columns provide excellent separations in a fraction of the time required by conventional particulate columns. The high permeability and porosity of the silica skeleton, and the resulting low back pressure allow for more flexible flow rates than particle-packed columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.

Chromolith® High Resolution HPLC Columns

Merck KGaA, Darmstadt, Germany

Chromolith® HighResolution: High resolution separations without ultra high pressure. The revolutionary monolithic Chromolith® HighResolution columns are made from a single rod of high-purity monolithic silica, rather than from particles. Their unique bimodal pore structure allows the chromatographic performance to be improved significantly. Speed of analysis (separations up to 9 times faster if required) Separations two times faster at half the column back-pressure compared to μm columns Higher sample throughput Improved HPLC system security Significantly increased column lifetime Reduced maintenance on HPLC pump and injector seals Reduced need for sample preparation as columns are very resistant to blocking (even with biological samples) Column length no longer pressure limited Very high separation efficiency by column coupling

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.