ResourceSeparations

Chlormequat & Mepiquat analyzed with LCMS

24 Mar 2022

Analysis of Chlormequat and Mepiquat can be challenging since they are extremely hydrophilic and are only weakly retained in reversed-phase HPLC which makes their separation difficult. In addition, these compounds are retained too strongly in normal-phase (non-polar solvents) chromatography. Due to this situation, the preferred method of analysis for these compounds was ion-pair chromatography using high ionic strength (> 150mM) ion-pair reagents.

Cogent Diamond Hydride™ HPLC Columns

MicroSolv Technology Corp.

Cogent Diamond Hydride HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a very small amount of carbon on the surface that adjusts the column hydrophobicity to an important level. This makes these columns have unique separation capabilities for scientists working in LCMS, metabolomics, bioanalytical, amino acids, organic acids, carbohydrates, and very polar small molecules.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Chlormequat & Mepiquat analyzed with LCMS