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Characterization of high-resolution carrier ampholytes

Isogen Life Science
14 Oct 2019

Since its inception, iCIEF has become a key technique for analyzing protein charge heterogeneity. Carrier ampholyte quality dictates the reproducibility of the separation itself as well as the potential for downstream assays through fractionation or iCIEF-MS coupling. A lack of consistency between CA lots from a single manufacturer can introduce significant problems in the application of CIEF as a reproducible tool in the biopharmaceutical industry, particularly in quality control.

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CEInfinite

Isogen Life Science

CEInfinite Preparative facilitates charge variant characterization by direct MS-detection and automated fractionation. CEInfinite is a whole column imaging CIEF, which allows fast method development and high throughput compared to traditional CE. The system is known for its robustness and is FDA CFR 21 Part 11 compliant

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.Protein SeparationElectrophoresis

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Characterization of high-resolution carrier ampholytes