ResourceLife Sciences
Characterization of High Concentration Protein Solutions with the pUNk
4 Jan 2018Dynamic light scattering (DLS) is widely used to detect the size and distribution of molecules, particles and their aggregates under a variety of solvent conditions. In the field of protein crystallography, the process of increasing the concentration of protein solutions required for crystallization often encourages unwanted aggregation. However, some DLS systems are incapable of detecting these aggregates due to excessive light scattering from the concentrated sample.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.AggregationProtein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.Dynamic Light ScatteringDynamic light scattering is used to measure the size distribution of particles in suspension by analyzing the way light scatters off them. It is commonly used in materials science, nanotechnology, and protein characterization. Browse our peer-reviewed product directory to find the best dynamic light scattering systems, compare products, check reviews, and get pricing directly from manufacturers.