Accurate automated cell counting and viability analysis of trypan blue-stained PBMCs using the LUNA-III™ Cell Counter

24 Feb 2025

Staining solutions like trypan blue can further enhance detection accuracy by making cell edges more visible. This approach aims to improve the accuracy and precision of PBMC counting and viability assessments, providing a reliable yet affordable solution. This application note validates the effectiveness of the LUNA-III™ automated cell counter by comparing its PBMC data with the LUNA-FX7™ automated cell counter.

LUNA-III™ Automated Cell Counter

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The LUNA-III™ Automated Cell Counter provides unmatched accuracy with advanced algorithms, precise autofocusing, reusable slides, non-toxic dyes, network access, re-analysis options, and a compact, user-friendly design.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.Fluorescent Dyes
Cell counting & viability analysis of trypan blue-stained PBMCs using LUNA-III