ResourceLife Sciences
Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways
1 May 2013Wnt signaling has been extensively investigated as an effector of diverse physiological processes including neuronal development and plasticity, bone development and formation, as well as pathological diseases, such as cancer and neurodegeneration. In this application note the Screen-Well® Autophagy compound library was screened using the Leading Light™ Wnt Reporter Assay Kit. Sets of Wnt agonists and antagonists were identified among the compounds. Most of the Wnt antagonists identified by this screen are known to be positive modulators of autophagy pathway or mitochondrial poisons.
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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. 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