ResourceLife Sciences
Cationic polymer transfections in Corning HYPERFlask cell culture vessels
14 Jun 2020One of the most useful tools in cell biology research is transfection, the introduction of foreign DNA into eukaryotic cells. In this study, HeLa and CHO-K1 cells were transfected using Polyplus-transfection jetPEI™ transfection reagent along with two different DNA constructs: one designed to test transfection via secreted protein production and the other to determine transfection efficiency through GFP expression within cells.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.Lab EssentialsLab essentials include laboratory consumables that are vital to the operation of all laboratories.