ResourceLife Sciences
Cardiomyocyte Beating and Cytotoxicity Assays on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer
14 Nov 2014This application note describes a cardiac beating assay using the SpectraMax i3 Multi-Mode Microplate Platform and the EarlyTox™ Cardiotoxicity Kit from Molecular Devices® for assessment of compound effects upon cardiomyocytes. Cardiomyocyte beating parameters were measured on the SpectraMax i3 Platform using a kinetic, well-by-well fluorescence read mode and analyzed using Peak Pro software algorithms in SoftMax® Pro Software. Sixteen analysis parameters, including peak count, peak frequency, peak amplitude, peak width, decay time, and several others, were calculated.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. 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