ResourceLife Sciences
cAMP/IP-One HTplex™ Cell-Based Experiment Performed on Mithras LB 940 using HTRF<sup>®</sup> Technology
25 Apr 2017This application note describes the cAMP and IP1 HTplex™ competitive immunoassay which can detect both cAMP and IP1 simultaneously by using two different acceptors. It describes the assay principle, protocol and results. The Mithras LB 940, a multimode plate reader, is equipped with an additional emission filter to detect acceptor emission. The application note concludes that this assay produces reliable results, as evidenced by the excellent Z values and the comparison of the results to that found in single analyte assays.
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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. 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