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Calcium 3 Assay Kit: An Optimal No-Wash Calcium Indicator Reagent for Non-Optical Calcium Responses

Molecular Devices®
24 Oct 2012

Fluorescent dye indicators for intracellular calcium measurements are widely used tools in biology, in particular in the field GPCR pharmacology and functional characterization. In this application note the several GPCRs were transiently transfected into human embryonic kidney cells (HEK-293) at non-optimal conditions, specifically the cells were seeded at modest over-confluence at the time of transfection. Subsequently these cells were tested with the FLIPR® Calcium 3 no-wash reagent from Molecular Devices to measure agonist-stimulated calcium signaling. Use of Calcium 3 improved data quality as evidenced by an increase in signal dynamic range, improvement in well-to-well variability and virtual elimination of dilution artifact following compound addition.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CalciumFluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.gPCRHEK CellsDyes

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Calcium 3 Assay Kit: An Optimal No-Wash Calcium Indicator Reagent for Non-Optical Calcium Responses