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Brownlee Superficially Porous Particle (SPP) HPLC Columns
8 May 2012This note introduces Superficially Porous Particle (SPP) columns. It outlines the advantages of Brownlee SPP columns over traditional HPLC columns such as reduced Eddy diffusion and reduced solute mass transfer. A performance comparison is made between SPP and 5μm columns as well as SPP and sub-2μm UHPLC columns.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solute Mass TransferEddy DiffusionSuperficially Porous Particle