ResourceLife Sciences
Biometra TAdvanced & qTOWERiris
19 Feb 2024In this application note, explore the preparation of DNA amplicon sequencing libraries for NGS using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs GmbH) on the Biometra TAdvanced 96 SG and performed library quantification using the real-time thermal cycler qTOWERiris.
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qTOWER iris: The Real-Time PCR-Thermocycler
Analytik Jena GmbH+Co. KGNo matter where your journey takes you in the world of qPCR, this real-time PCR thermal cycler is ready for anything, giving you full control and freedom of choice - from consumables to assays to colour modules. The instrument is operating quietly, smoothly and delivers reliable results on six channels. The temperature and readout accuracy are unsurpassed. With this open system you are well equipped for all your projects.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.