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Bioinformatics Pipeline for ChIP-Seq Analyses

Diagenode
28 May 2015

This poster presents a modular bioinformatics pipeline which was developed specifically for the analysis of ChIP-seq data, and can be easily modified and extended to accommodate other high-throughput sequencing and epigenetic applications, such as RNAseq or methylation analysis and other custom data processes.

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High Resolution ChIP-seq Library Prep kit

Diagenode

Diagenode's High Resolution Library Preparation Kit significantly enhances the resolution of transcription factor binding sites to single nucleotide levels.Using an optimized, proprietary protocol using an exonuclease to digest protein-bound DNA from the 5' end to within a few nucleotides of the protein binding site, the precise location of transcription factor-bound DNA can be determined. The High Resolution Library Preparation Kit provides a much higher resolution than in ChIP-seq and thus more accurately determines binding sites.Features: Ultra-precise: Provides exact binding sites for transcription factors Higher resolution and improved signal-to-noise ratio Optimized for use with the iDeal ChIP-seq kit for Transcription Factors Compatible with Illumina sequencing Multiplex up to 8 samples

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Chem / BioinformaticsCheminformatics and bioinformatics are computational techniques used in chemistry and biology, respectively, for data acquisition, processing and storage. Cheminformatics focuses on compound information, whereas bioinformatics is mainly applied to analysis and modeling of genomics, genetic and sequencing information. Hardware and software is available for data acquisition, analysis, management and storage.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Epigenetic AnalysisEpigenetic analysis products are used to study heritable phenotype changes that do not alter the DNA sequence, such as DNA modifications affecting gene activity. Determine whether genes have been methylated with DNA methylation quantification kits or methylation microarrays. Assess epigenetic regulation with histone modification kits or ChIP-on-chip microarrays. Kits are also available for bisulfite conversion and amplification of your samples, as well as epigenomic library preparation kits for next-generation sequencing (NGS). Find the best epigenetic products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChIP-SequencingBioinformaticsBioinformatics uses computational models and methodologies to study biological processes and to understand biological data.

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Bioinformatics Pipeline for ChIP-Seq Analyses