ResourceLife Sciences
Bio-Rad Fluorescent Western Blotting Antibodies Product Brochure
26 Jun 2019This product brochure details the key advantages of using StarBright™ Blue 700 Secondary Antibodies in western blotting.
Related products
Request Quote for All Products
StarBright™ Blue 700 Fluorescent Secondary Antibodies
Bio-RadThe StarBright Blue 700 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 470 nm and emission maximum at 705 nm. StarBright Blue 700 is a brighter alternative to PerCP-Cy5.5, with less spillover, and does not exhibit tandem breakage.
Links
Tags
AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.Secondary Antibodies