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Better Gel Filtration: A New Look at Method Development Parameters Using Yarra™ GFC Columns

Phenomenex Inc
12 Jan 2015

An affordable, high efficiency Yarra GFC column was recently introduced and is significantly more efficient than other GFC columns on the market. In addition to higher efficiency, Yarra columns demonstrate significantly higher inertness to ionic interactions versus other GFC columns; however, such chemical characteristics require optimization to operating parameters. Performing method development through mobile phase composition using next-generation Yarra GFC columns will be discussed.

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Yarra Ultra-High Efficiency Aqueous GFC/SEC Columns

Phenomenex Inc

Starting with 3 µm ultra-pure silica, Yarra particles are densely bonded with a proprietary surface chemistry. Coupled with tight particle and pore size distribution as well as strict packing and QC specifications, Yarra GFC columns allow for the highest efficiencies and resolution possible. Yarra columns are offered in three phases (SEC-2000, 3000, and 4000), and are ideal for the separation of small to large proteins, as well as biological therapeutics and biosimilars.

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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Better Gel Filtration: A New Look at Method Development Parameters Using Yarra™ GFC Columns