Best practice for nucleic acid thermal stability measurements using the Cary 3500 UV-Vis Spectrophotometer

The Cary 3500 Flexible UV-Vis is a double beam spectrophotometer with superior photometric performance—for challenging measurements with minimal sample preparation—that can be deployed in regulated environments. The system offers a unique large sample compartment with small footprint for analyzing liquid samples that require long pathlength cuvettes, as well as characterizing solid samples. A xenon flash lamp—complete with 10-year replacement warranty (for Cary 3500 instruments purchased from Agilent or participating partners)—eliminates the daily warm-up time and drastically reduces the frequency and cost of lamp replacement. The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database. Features: Measure liquid samples via unique variable pathlength rectangular and cylindrical cell holders, which enable quick and reproducible pathlength changes up to 10 cm. This design is toolless and eliminates time-consuming alignment procedures.  Handle a wide variety of solid samples and thin films with a large sample compartment that accommodates most sample sizes while minimizing its footprint on the lab bench. Ensure you never miss a data point—even in rapid reaction kinetics measurements—with advanced xenon flash lamp technology achieving a data collection rate of 250 points per second. Achieve research grade photometric performance, stability, and high linear dynamic range with minimal sample preparation. Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely. Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstation’s Help and Learning Center. Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPA’s 40 CFR Part 160. Store and manage your Cary 3500 Flexible UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location. Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities. This solution received My Green Lab’s ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.

The Cary 3500 Compact UV-Vis is a double beam spectrophotometer featuring new and unique measurement capabilities that can be deployed in regulated environments. The system performs ambient and temperature-controlled experiments with air-cooled Peltier temperature control, and it accurately analyzes low volume samples—providing in-depth analysis by collecting up to 250 data points per second. The xenon flash lamp—complete with 10-year replacement warranty (for Cary 3500 instruments purchased from Agilent or participating partners)—eliminates the daily warm-up time and drastically reduces the frequency and cost of lamp replacement. The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database. Features: Perform accurate and quick temperature-controlled experiments from 0 to 110 °C using a unique and fully integrated air-cooled Peltier system. Control experimental temperature from inside the cuvette using highly accurate, solid-state digital Cary temperature probes with thermal ramp rate up to 40 °C/min. Ensure accurate analysis of small-volume measurements with a highly-collimated and uniform beam of less than 1.5 mm width, coupled with permanent optical alignment of the stationary cell holders. Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely. Achieve research grade photometric performance, stability, and range with a double beam spectrophotometer featuring leading optical technologies. Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstation’s Help and Learning Center. Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPA’s 40 CFR Part 160. Store and manage your Cary 3500 Compact UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location. Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities. This solution received My Green Lab’s ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.

The Cary 3500 UV-Vis double beam spectrophotometer offers new and unique measurement capabilities in ultraviolet-visible spectroscopy—for routine analysis and challenging measurements, including advanced data integrity in compliant environments. Measure up to four temperature experiments, across eight cuvette positions, simultaneously—with air-cooled Peltier temperature control. Xenon flash lamp technology enables accurate monitoring of kinetic experiments—with a data collection rate up to 250 points per second. You can also analyze low volume samples accurately, and reproducibly—every time. The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database Features: Measure samples at four temperatures across eight cuvette positions in a single experiment and collect data from each cuvette simultaneously—dramatically reducing analysis time with the Cary 3500 double beam spectrophotometer. Accurately and quickly control the temperature of your samples from 0 to 110 °C without water, noise, or messy cables using a unique and fully integrated air-cooled Peltier system. Control UV-Vis thermal ramp measurements at up to 30 °C/min using highly accurate, solid-state digital Cary temperature probes that control experimental temperature from inside the cuvette. Ensure analytical accuracy for small-volume measurements with a highly-collimated and uniform beam of less than 1.5 mm width, coupled with permanent optical alignment of the stationary cell holders. Get your double beam spectrophotometer results faster than a diode array, with advanced xenon flash lamp technology achieving a data collection rate of 250 points per second. Achieve research grade photometric performance, stability, and range with the Cary 3500 double beam spectrophotometer. Streamline method setup with the Cary UV Workstation software for scanning, concentration, kinetics, and thermal control. The software includes a Help and Learning Center with intuitive video tutorials to get you up and running fast. Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as and ISO/IEC 17025 and EPA’s 40 CFR Part 160. Store and manage Agilent Cary 3500 UV-Vis data in a local secured storage on the workstation PC, or in a central storage location. The OpenLab software suite includes optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities. This solution received My Green Lab’s ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.