ResourceLife Sciences
Basic Considerations for Bioluminescence Resonance Energy Transfer (BRET) Assays for G-protein Coupled Receptor Protein Interactions in Living Cells
20 Jun 2013BRET has been successfully applied to the study of GPCR homo- and heterodimerization as well as receptor/β-arrestin interactions involved in receptor desensitisation and trafficking in mammalian cells. The main advantage of BRET is that it allows protein-protein interactions to be monitored in real-time, in their correct location, in live cells. In this application note read about the basic considerations for a BRET assay when studying protein interactions in living cells.
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.BRETBioluminescence